Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta2. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TGF-beta2. A competitive inhibition reaction is launched between TGF-beta2 (Standards or samples) and biotin-conjugated TGF-beta2 with the pre-coated antibody specific for TGF-beta2. The more amount of TGF-beta2 in samples, the less antibody bound by biotin-conjugated TGF-beta2. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of TGF-beta2 in the sample. The color development is stopped and the intensity of the color is measured.
Background: Transforming growth factor-beta 2 (TGF- beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. There are two named isoforms of this protein, created by alternative splicing of the same gene. TGF- beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF- beta 2 influences cultured trabecular meshwork cells.TGF- beta 2 reduced outflow facility when perfused into cultured human anterior segments. Furthermore, TGF- beta 2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility. Although the distribution of accumulated fibrillar material was different in these perfused eyes than that in POAG, the difference could have been due to variation in biomechanical environment for trabecular meshwork cells in cultured anterior segments compared with the living eye. elevated TGF- beta 2 levels in the aqueous humor played a role in the pathogenesis of the ocular hypertension in POAG